primary normal heart cardiac fibroblasts  (Lonza)

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: Exosome nanoparticle tracking analysis using NanoSight NS300 system. Five videos (60 s duration each) of Brownian motion of nanoparticles were recorded and analyzed. The samples were measured with manual shutter and gain adjustments (N = 3 in each group). (A). Control fibroblast exosomes. (B). TGF-β fibroblast exosomes. (C) HF fibroblast exosomes.

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: A . HF target panel genes differentially expressed in TGF-β treated fibroblasts and their exosomes (N=3 in each group) . Genes that were overlapped in both fibroblasts and exosomes and were differentially expressed in TGF-β treated compared to control, up-regulated (bold) and down-regulated (italic) and opposite expression level (bold-italic). B. Genes that were differentially expressed in fibroblasts treated with TGF-β compared to control but not in exosomes and that were differentially expressed in exosomes but not in the cells.

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control, Expressing

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: IPA of the differentially expressed genes in fibroblasts exosomes treated with TGF-β compared to control with activation Z-scores. (A). Hypertrophy of the heart (2.198) and cardiac muscle signals (2.432). (B) and (C) mi-RNA 155 (1.138) and 21 (0.88) Z-score.

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control, Activation Assay

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: Collagen gel contraction (N = 6 in each group). Control and HF fibroblasts were treated ad cast into collagen gels and maintained in floating culture in serum-free media. The size of gels was measured on day 1, 2 and 3 and shown as percentage of initial area. P + T represents control fibroblasts treated with both PGE and TGF-β.

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: (A). Multidimensional scaling plot of control cardiomyocytes with no exosomes (CNE), co-cultured cardiomyocytes with control fibroblast exosomes (CFE), TGF-β treated fibroblast exosomes (TGFE) and HF fibroblasts exosomes (HFE), (N = 3 in each group). (B). Venn diagram of differentially expressed cells in co-cultured cardiomyocytes with TGF-β treated fibroblast exosomes (TGFE) and HF fibroblasts exosomes (HFE) compared to control fibroblast exosomes (CFE).

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control, Cell Culture

Journal: Heliyon

Article Title: TGF-β induces a heart failure phenotype via fibroblasts exosome signaling

doi: 10.1016/j.heliyon.2019.e02633

Figure Lengend Snippet: A. Target panel genes that were up-regulated in both TGF-β treated fibroblast exosomes and HF fibroblasts exosomes compared to control fibroblast exosomes. B. Target panel genes that were down-regulated in both TGF-β treated fibroblast exosomes and HF fibroblasts exosomes compared to control fibroblast exosomes.

Article Snippet: Primary normal heart cardiac fibroblasts were purchased from Lonza (Walkersville, MD).

Techniques: Control